Библиографическое описание:The Hybrid Protein ZZ–OL as an Analytical Tool for Biotechnology Research : научное издание / V. V. Krasitskaya [et al.]. - Текст : непосредственный // Russian Journal of Bioorganic Chemistry. - 2020. - Т. 46, № 6. - P. 1004-1010. - ISSN 1068-1620. - ISSN 1608-330X, DOI 10.1134/S106816202006014X.
Аннотация:The gene of the hybrid protein that encodes the double synthetic fragment proZZ of the immunoglobulin-binding domain of protein A of Staphylococcus aureus and apo-obelin joined by a short linker has been cloned. The corresponding hybrid protein has been obtained by expression in Esсheriсhia coli cells. The protein activated with a substrate (coelenterazine) possesses the bioluminescent Са<sup>2+</sup>-dependent activity of the photoprotein close to that of recombinant wild-type obelin, and the immunoglobulin-binding ability of protein A. It has been shown that the hybrid can be used as a highly sensitive label to detect antibodies and estimate their affinity and interaction with recombinant proteins, as well as in investigations of other kinds.
Издательство:Pleiades Publishing, Ltd. (Плеадес Паблишинг, Лтд), Москва
Источник:Russian Journal of Bioorganic Chemistry
Выпуск:Т. 46, № 6
Номера страниц:1004-1010
Держатель оригинала документа:Institute of Biophysics,Federal Research Center,Krasnoyarsk Research Center,Siberian Branch,Russian Academy of Sciences, Institute of Chemical Biology and Fundamental Medicine,Siberian Branch,Russian Academy of Sciences
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