Библиографическое описание:Mechanism and color modulation of fungal bioluminescence : научное издание / Zinaida M. Kaskova [et al.]. - Текст : непосредственный // Science Advances. - 2017. - Т. 3, № 4. - P. e1602847. - ISSN 2375-2548, DOI 10.1126/sciadv.1602847.
Аннотация:Bioluminescent fungi are spread throughout the globe, but details on their mechanism of light emission are still scarce. Usually, the process involves three key components: an oxidizable luciferin substrate, a luciferase enzyme, and a light emitter, typically oxidized luciferin, and called oxyluciferin. We report the structure of fungal oxyluciferin, investigate the mechanism of fungal bioluminescence, and describe theuseof simple synthetic alpha-pyrones as luciferins to produce multicolor enzymatic chemiluminescence. A high-energy endoperoxide is proposed as an intermediate of the oxidation of the native luciferin to the oxyluciferin, which is a pyruvic acid adduct of caffeic acid. Luciferase promiscuity allows the use of simple alpha-pyrones as chemiluminescent substrates.
