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- proach tested was lowering of the expression temperature (pCold ex- pression system). Although it facilitated the proper folding of G. prin- ceps luciferase, it simultaneously slowed down the rate of protein synthesis [15]. The attempts to express copepod...
(Invitrogen) cul- tured in suspension at 28 °C without CO2 using serum-free medium Sf900 II SFM (Life Technologies) were infected with the P2 viral stock (titer ~5 × 107 infectious units per 1 mL) at a multiplicity of infection (MOI) of 2 plaque-forming units...

, In Economic Journal, 1931, 41 (162), 173– 198. Available at: https://www.jstor.org/stable/2223697?origin=crossref Karasmanaki E., Ioannou K., Katsaounis K., Tsantopoulos G.  The attitude of the local community towards investments in lignite before...
, 2006, 407 p. Lomakina  N. V.  Complex exploitation of the Far East mineral resources: effects “new model” of the macroregional development, In E 3S Web of Conferences, 2020, 192, 03006, аvailable at: https://doi. org/10.1051/e3sconf/202019203006 Luo Yu...

be inefficient for Cys- rich proteins [16] which GpLuc belongs to. The other tested approaches aimed at enhancing GpLuc solubility in E. coli. These included lowering of the expression temperature (pCold expression system) [17] and expression of GpLuc as a...
Sf9 cells, the Bac-to-Bac Baculovirus expression system (Invitrogen) was used. The GpLuc coding sequence with native signal peptide was amplified using pCMV-GLuc template (NanoLight Technology) with humanized Gaussia luciferase and specific primers...

случаев (P <0,05). Было предложено пороговое значение сурвивина 17,7 пг/мл с приблизительной чувствительностью 82,9% и специфичностью 81,1% (Р <0,0001), тогда как цитология мочи имела чувствительность 66,7% и специфичность 96,0%. Среди опубликованных...
Европейцы СС vs GG ОШ p-значение GC vs GG ОШ p-значение 1.77 0.004 0.97 0.853 1.42 0.008 0.85 0.579 1.65 <0.001 1.37 0.008 2.02 1.54 <0.001 0.045 1.26 0.97 0.112 0.953 В отношении рака мочевого пузыря для азиатских популяций показана связь...

by SignalP 4.0 program (23). The mammalian (8,14,21) and insect cells (11,24) were found to efficiently secrete all the investigated copepod luciferases expressed with natural signal peptides. For example, the percentage of secreted proteins in culture...
medium of HEK293 cells transiently transfected with luciferases from M. okhotensis and P. abdominalis Page 6 of 65Photochemistry and Photobiology For Peer Review 7 was ~98-99% (14). The GpLuc secretion in human cells directed by its own native signal...

.Frolenkov On the solvability of the system of two multidimensional . . . 1. Statement of the problem In the space Zn of variables x)P : : : P xn choose ri different points �iki , (ki 5 )P ri) for each variable xi (i 5 )P n): In the strip G[(NT ] 5 {(tP x...
)|( 6 t 6 iP x 5 (x)P : : : P xn) ∈ Zn} consider the Cauchy problem for the system of loaded nonclassical parabolic equations ut(tP x) 5 n∑ i=) vi)(tP <u(t)P <v(t))uxixi + n∑ i=) wi)(tP <u(t)P <v(t))uxi + f)(tP xP uP vP <u(t)P <v(t))P vt(tP x) 5 n∑ i...

,ЖОРДАНОВАМАТ- РИЦА. Цель работы — определить групповые порядки матричных унипотентных элементов над основным полем K характеристики p отличной от нуля. В результате исследований найдены групповые порядки всех клеток жордана для небольших n и p, где n — размерность...
клетки жордана, а p — характеристика основ- ного поля. Сформулированы и доказаны леммы : о групповом порядке унипотентной жордановой клетки при p ≥ n ; о групповом порядке унипотентной жордановой матри- цы, состоящей из унипотентных жордановых клеток...