An Agrobacterium-mediated transient gene expression system for intact leaves

J Kapila, R De Rycke, M Van Montagu, G Angenon - Plant science, 1997 - Elsevier
An efficient and reproducible Agrobacterium-mediated transient gene expression system for
intact leaf tissue was developed. A high level of transient expression was observed when …

The effect of temperature on Agrobacterium tumefaciens‐mediated gene transfer to plants

W Dillen, J De Clercq, J Kapila, M Zambre… - The plant …, 1997 - Wiley Online Library
Agrobacterium tumefaciens is generally used to achieve genetic transformation of plants.
The temperatures that have been used for infection with Agrobacterium in published …

High level accumulation of single‐chain variable fragments in the cytosol of transgenic Petunia hybrida

…, C De Wilde, A Jacobs, J Kapila… - European Journal of …, 1999 - Wiley Online Library
The accumulation of five murine single‐chain variable fragments, binding to dihydroflavonol
4‐reductase, was analyzed in transgenic Petunia hybrida plants. The five scFv‐encoding …

Higher-plant plasma membrane cytochromeb 561: A protein in search of a function

H Asard, J Kapila, W Verelst, A Bérczi - Protoplasma, 2001 - Springer
During the past twenty years evidence has accumulated on the presence of a specific high-potential,
ascorbate-reducibleb-type cytochrome in the plasma membrane (PM) of higher …

Tissue‐specific expression and developmental regulation of cytochrome b561 genes in Arabidopsis thaliana and Raphanus sativus

W Verelst, J Kapila, J de Almeida Engler… - Physiologia …, 2004 - Wiley Online Library
Ascorbate (Asc) is an essential molecule in many aspects of development and stress
responses in plants and animals. Cytochromes b561 (cyts b561) are tightly coupled to Asc …

Strategies for improving the nutritional quality of Phaseolus beans through gene engineering

…, J De Clercq, W Dillen, A Goossens, J Kapila… - BASE, 1999 - popups.uliege.be
… Geert Angenon (1), Janniek De Clercq (1), Willy Dillen (1), Alain Goossens (1,2), Jyoti
Kapila (1), Mukund Zambre (1), Marc Van Montagu (1) … D illen W., De C lercq J., Kapila J …

In vitro regeneration and Agrobacterium-mediated transformation of Phaseolus vulgaris L.(common bean) and P. acutifolius A. Gray (tepary bean)

…, J De Clercq, A Goossens, J Kapila… - … Congress, Part 11 …, 1998 - actahort.org
Dry Phaseolus beans are an important staple food and immature bean pods are popular as
a vegetable. Phaseolus seeds are deficient in the sulfur-containing amino acids and the …

Host Range of an Insecticidal Crystal Protein of Bacillus thuringiensis subsp. kurstaki Produced in Escherichia coli

P Ratnakar, N Vijayalakshmi, J Kapila… - Journal of Plant …, 1994 - Springer
A crylA(c)-like gene of Bacillus thuringiensis subsp. kurstaki strain HD1 was over-expressed
in Escherichia coli from a multicopy plasmid. Biological toxicity tests conducted on the …

Agrobacterium mediated transient gene expression in leaves of Phaseolus vulgaris

J Kapila, M van Montagu, G Angenon - Biotecnologia Aplicada - tspace.library.utoronto.ca
Transient gene expression (TGE) assays have two important advantages as compared to
stable transformation; gene expression can be measured very shortly after DNA delivery and is …

[PDF][PDF] An zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA Agrobacterium-mediated transient gene intact leaves

J Kapila, R De Rycke, M Van Montagu, G Angenon - Plant Science, 1997 - academia.edu
Phasr olus zyxwvutsrqponmlkjihgfedcbaZYXWVUTSRQPONMLKJIHGFEDCBA wlgaris leaves.
but similar results were obtained with other plant species. such as P/mroh~. s uc, utifdim. …