Determination of glycosylated hemoglobin by affinity chromatography: comparison with colorimetric and ion-exchange methods, and effects of common interferences.

…, RI Krohn, EK Fujimoto, AK Mallia… - Clinical …, 1982 - academic.oup.com
An affinity-chromatographic method for determination of glycosylated hemoglobin (Anal. Lett.
14: 649-661, 1981) is compared with the thiobarbituric acid colorimetric (I) (Clin. Chem. 27: …

Preparation and use of a boronic acid affinity support for separation and quantitation of glycosylated hemoglobins

AK Mallia, GT Hermanson, RI Krohn… - … Letters, Part B …, 1981 - Taylor & Francis
Cross-linked agarose activated with carbonyl diimidazole and subsequently coupled to
aminophenyl boronic acid provides a highly efficient matrix for separation and quantitation of …

Affinity chromatographic purification of immunoglobulin M antibodies utilizing immobilized mannan binding protein

JR Nevens, AK Mallia, MW Wendt, PK Smith - Journal of Chromatography A, 1992 - Elsevier
A method is described for the rapid and efficient affinity chromatographic purification of murine
monoclonal immunoglobulin M (IgM) which utilizes immobilized rabbit mannan binding …

Praziquantel Affects the Regulatory Myosin Light Chain of Schistosoma mansoni

M Gnanasekar, AM Salunkhe, AK Mallia… - Antimicrobial agents …, 2009 - journals.asm.org
Praziquantel (PZQ) is the drug of choice for schistosomiasis and probably is the only highly
effective drug currently available for treating schistosomiasis-infected individuals. The mode …

Metabolism of retinol-binding protein and vitamin A during hypervitaminosis A in the rat

AK Mallia, JE Smith, DW Goodman - Journal of lipid research, 1975 - Elsevier
Vitamin A is normally transported in plasma as retinol bound to a specific protein, retinol-binding
protein (RBP). Detailed studies were conducted to examine the effects of excess vitamin …

Site-directed immobilization of proteins

PL Domen, JR Nevens, AK Mallia… - … of Chromatography A, 1990 - Elsevier
To determine if immobilization chemistry can be used to orient antibody on a support so that
the bivalent binding potential can be fully utilized, we developed three activated matrices …

A high-affinity reversible protein stain for Western blots

BS Antharavally, B Carter, PA Bell, AK Mallia - Analytical biochemistry, 2004 - Elsevier
We describe a reversible staining technique, using MemCode, a reversible protein stain by
which proteins can be visualized on nitrocellulose and polyvinylidine fluoride (PVDF) …

Solid‐phase biotinylation of antibodies

E Strachan, AK Mallia, JM Cox… - Journal of Molecular …, 2004 - Wiley Online Library
Biotinylation is an established method of labeling antibody molecules for several applications
in life science research. Antibody functional groups such as amines, cis hydroxyls in …

Quantitation of proteins using a dye–metal-based colorimetric protein assay

BS Antharavally, KA Mallia, P Rangaraj, P Haney… - Analytical …, 2009 - Elsevier
We describe a dye–metal (polyhydroxybenzenesulfonephthalein-type dye and a transition
metal) complex-based total protein determination method. The binding of the complex to …

Efficient removal of detergents from proteins and peptides in a spin column format

BS Antharavally, KA Mallia, MM Rosenblatt… - Analytical …, 2011 - Elsevier
Detergents are commonly used in protein–chemistry protocols and may be necessary for
protein extraction, solubilization, and denaturation; however, their presence interferes with …