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Plant Photomorphogenesis

Methods and Protocols

  • Book
  • © 2021

Overview

Editors:
  1. Ruohe Yin

    1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

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  2. Ling Li

    1. Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

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    You can also search for this editor in PubMed   Google Scholar

  3. Kaijing Zuo

    1. School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

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  • Includes cutting-edge techniques
  • Provides step-by-step detail essential for reproducible results
  • Contains key implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2297)

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About this book

This book provides detailed protocols for research in plant photomorphogenesis. The collection includes a broad range of topics including assays for shade avoidance responses, assays for light-dependent protein-protein interactions, photobody detection with immunofluorescence and the super-resolution imaging method, protein complex isolation from plants, detection of homodimer and monomer of photoreceptor UVR8 with immunoblotting analysis, assays for seedling greening, procedures for studying skotomorphogenesis, phenotypic study of photomorphogenesis at the seedling stage, expression of Cryptochrome in insect cells, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Authoritative and practical, Plant Photomorphogenesis: Methods and Protocols serves as an ideal guide for researchers and students who are new to the field, as well as a stepping stone for experienced researchers to further their skills in this fast-developing field.

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Table of contents (18 protocols)

  1. Front Matter

    Pages i-xi
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  2. Setting Up an Arabidopsis LED Culture Module that Simulates Plant Neighbor Proximity

    • Carlos D. Crocco
    Pages 1-6

  3. Photobody Detection Using Immunofluorescence and Super-Resolution Imaging in Arabidopsis

    • Giorgio Perrella, Anna Zioutopoulou, Andrew Hamilton, Eirini Kaiserli
    Pages 7-19

  4. Analysis of Shade-Induced Hypocotyl Elongation in Arabidopsis

    • Yetkin Çaka Ince, Vinicius Costa Galvão
    Pages 21-31

  5. Isolation of UVR8 Protein Complexes

    • Yan Liu, Xi Huang
    Pages 33-40

  6. Phenotypic Study of Photomorphogenesis in Arabidopsis Seedlings

    • Chuanwei Yang, Famin Xie, Lin Li
    Pages 41-47

  7. Experimental Procedures for Studying Skotomorphogenesis in Arabidopsis thaliana

    • Huanhuan Jin, Hong Li, Ziqiang Zhu
    Pages 49-60

  8. Global Identification for Targets of Circadian Transcription Factors in Arabidopsis and Rice Using Chromatin Immunoprecipitation Followed by Sequencing (ChIP-seq)

    • Shuxuan Xu, Jing Huang, Jian Jin, Wei Huang
    Pages 61-74

  9. Co-immunoprecipitation Assays to Detect In Vivo Association of Phytochromes with Their Interacting Partners

    • Pengyu Song, Shaoman Zhang, Jigang Li
    Pages 75-82

  10. Detection of UVR8 Homodimers and Monomers by Immunoblotting Analysis in Tomato

    • Guoqian Yang, Xiaorui Liu, Li Lin
    Pages 83-93

  11. Characterization of Seedling Greening Process in Plant Photomorphogenesis

    • Wanqing Wang, Yuhong Li, Rongcheng Lin
    Pages 95-103

  12. Protoplast System for Studying Blue-Light-Dependent Formation of Cryptochrome Photobody

    • Xiangguang Lyu, Hongyu Li, Bin Liu
    Pages 105-113

  13. Uncover the Nuclear Proteomic Landscape with Enriched Nuclei Followed by Label-Free Quantitative Mass Spectrometry

    • Yan Wang, Zhuang Lu, Lei Wang
    Pages 115-124

  14. Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii

    • Huanling Yang, Fei Han, Yue Wang, Wenqiang Yang, Wenfeng Tu
    Pages 125-140

  15. Co-immunoprecipitation Assay for Blue Light-Dependent Protein Interactions in Plants

    • Jingyi Zhang, Shengbo He
    Pages 141-146

  16. Detecting Blue Light-Dependent Protein–Protein Interactions by LexA-Based Yeast Two-Hybrid Assay

    • Xiaolong Hao, Ling Li
    Pages 147-154

  17. Express Arabidopsis Cryptochrome in Sf9 Insect Cells Using the Baculovirus Expression System

    • Xu Li, Yawen Liu, Hongtao Liu
    Pages 155-160

  18. Semi-In-Vivo Pull-Down Assay for Blue Light-Dependent Protein Interactions

    • Xu Li, Yawen Liu, Hongtao Liu
    Pages 161-166

  19. Tobacco System for Studying Protein Colocalization and Interactions

    • Jingyi Zhang, Shengbo He
    Pages 167-174

  20. Back Matter

    Pages 175-176
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Editors and Affiliations

  • School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    Ruohe Yin, Kaijing Zuo

  • Key Laboratory of Urban Agriculture (South) Ministry of Agriculture, Plant Biotechnology Research Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai, China

    Ling Li

Bibliographic Information

  • Book Title: Plant Photomorphogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Ruohe Yin, Ling Li, Kaijing Zuo

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1370-2

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1369-6Published: 04 March 2021

  • Softcover ISBN: 978-1-0716-1372-6Published: 05 March 2022

  • eBook ISBN: 978-1-0716-1370-2Published: 03 March 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 176

  • Number of Illustrations: 12 b/w illustrations, 32 illustrations in colour

  • Topics: Plant Sciences, Plant Anatomy/Development

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