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Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones

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Abstract

Previous studies have shown that craniofacial bone marrow stromal cells (BMSCs) have a strong osteogenic potential. However, the mechanism by which BMSCs of various embryonic origins develop diverse osteogenic potentials remains unclear. To investigate the mechanisms regulating osteoblast differentiation in two different types of BMSCs, we compared the temporal and spatial mRNA and protein expression patterns of Satb2 and its downstream gene Hoxa2 by using real-time polymerase chain reaction, Western blotting and fluorescent immunostaining in mandible BMSCs (M-BMSCs) and tibia BMSCs (T-BMSCs) undergoing osteoblast differentiation. Higher levels of alkaline phosphatase, greater calcium accumulation and earlier expression of Runx2 were observed in osteogenic-induced M-BMSCs compared with T-BMSCs. Low levels of Satb2 were detected in both types of uninduced BMSCs but the majority of SATB2 was located in the nuclei of M-BMSCs. Notably, Satb2 was expressed earlier in M-BMSCs and Hoxa2, a downstream target of Satb2, was not expressed in uninduced M-BMSCs or during osteoblast differentiation, just as during embryonic mandible development. In contrast, Hoxa2 was reactivated in T-BMSCs during osteoblast differentiation. Based on these results, we conclude that SATB2 plays a different role during osteoblast differentiation of M-BMSCs and T-BMSCs. The earlier activation of Satb2 expression in M-BMSCs compared with T-BMSCs might explain the stronger osteogenic potential of M-BMSCs.

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Acknowledgements

We greatly appreciate the technical assistance of Jinhua Yu, Zilu Wang, Yangyu Zhen and Mifang Yang (Institute of Stomatology, School of Stomatology, Nanjing Medical University) with real-time PCR, Western blot and immunofluorescence.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Surgery, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, Jiangsu Province, People’s Republic of China

    Ping Zhang, Yu Fu, Tengfei Shan, Jinhai Ye, Yunong Wu, Zhenjiang Tao & Hongbing Jiang

  2. Institute of Stomatology, School of Stomatology, Nanjing Medical University, 136 Hanzhong Road, Nanjing, 210029, Jiangsu Province, People’s Republic of China

    Ping Zhang, Yu Fu, Tengfei Shan, Laikui Liu & Hongbing Jiang

  3. Department of Prosthodontics, Shanxi Red Cross Stomatology hospital, 18 Xinghualing, Taiyuan, 030001, Shanxi Province, People’s Republic of China

    Jie Men

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  1. Ping Zhang
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Correspondence to Laikui Liu or Hongbing Jiang.

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This work was supported by a National Natural Science Foundation of China grant (81070810) and a Foundation of Jiangsu Educational Committee grant (09KJB320003) and included a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.

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Zhang, P., Men, J., Fu, Y. et al. Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones. Cell Tissue Res 350, 425–437 (2012). https://doi.org/10.1007/s00441-012-1487-4

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