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NaCl induced cross-acclimation to UV-B radiation in four Barley (Hordeum vulgare L.) cultivars

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Abstract

The effect of pre-treatment with 200 mM NaCl on the response of four barley cultivars (Hordeum vulgare L. cv. Bülbül-89, Kalaycı-97, Tarm-92 and Tokak-157/37) to UV-B radiation was investigated. Salt stress as well as UV-B irradiation led to a decrease of the total chlorophyll (chl) content in all cultivars, except in Kalaycı-97. While carotenoids are almost not affected by NaCl treatment, UV-B irradiation caused an increase by 5–20% of carotenoid content of all cultivars. UV-B induced damages of photosynthetic apparatus were estimated by the rate of photosynthetic electron transport measured by chl fluorescence and the rate of oxygen evolution, the latter being more affected. Pre-treatment with NaCl alleviated harmful effect of UV-B irradiation on F v/F m and ETR, but not on oxygen evolution. UV-B-induced and UV-B-absorbing compounds with absorption at 300 and 438 nm increased as a result of UV-B treatment. The level of stress marker proline increased considerably as a result of NaCl treatment, while UV-B irradiation resulted in a pronounced increase of the level of H2O2. MDA enhanced in the seedlings subjected to salt and UV-B stress. Established cross-acclimation to UV-B as a result of salt treatment could be due to the increased free proline and the level of UV-B absorbing compounds in barley seedlings subjected to NaCl.

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Abbreviations

Chl:

Chlorophyll

ETR:

Electron transport rate

Fo and Fm:

Initial and maximum fluorescence yield

MDA:

Malondialdehyde

PPFD:

Photosynthetic photon flux density

PSII:

Photosystem II

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

Tris:

Tris[hydroxymethyl]-aminomethane

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Acknowledgments

This research was supported by The Scientific and Technological Researched Council of Turkey, Project number, TBAG-U/135 and Bulgarian Academy of Sciences.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Hacettepe University, 06800, Beytepe, Ankara, Turkey

    Hüsnü Çakırlar & Nuran Çiçek

  2. Academic Metodi Popov Institute of Plant Physiology, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street, Building 21, Sofia, 1113, Bulgaria

    Ivanka Fedina & Katya Georgieva

  3. Department of Biology, Faculty of Art and Sciences, Sakarya University, Esentepe Campus, 54187, Sakarya, Turkey

    Ali Doğru

  4. Institute of Biophysics, Bulgarian Academy of Sciences, Academic Georgi Bonchev Street, Building 21, Sofia, 1113, Bulgaria

    Maya Velitchkova

Authors
  1. Hüsnü Çakırlar
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  2. Nuran Çiçek
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  3. Ivanka Fedina
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  4. Katya Georgieva
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  5. Ali Doğru
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Corresponding author

Correspondence to Nuran Çiçek.

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Communicated by F. Corbineau.

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Çakırlar, H., Çiçek, N., Fedina, I. et al. NaCl induced cross-acclimation to UV-B radiation in four Barley (Hordeum vulgare L.) cultivars. Acta Physiol Plant 30, 561–567 (2008). https://doi.org/10.1007/s11738-008-0155-5

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  • DOI: https://doi.org/10.1007/s11738-008-0155-5

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