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Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824)

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Abstract

An 8-week feeding trial was conducted to evaluate the effect of replacing fish meal with pumpkin seed meal (PSM) or phosphorylated protein concentrate of pumpkin seed meal (PPCPS) on growth and metabolic responses of silver catfish. Five isonitrogenous and isocaloric diets were formulated. Control diet contained fish meal as the main protein source. The treatment groups contained 25 and 50 % of either PSM or PPCPS protein replaced the fishmeal protein. A total of 400 silver catfish, with initial mean weight of 24 ± 0.46 g, were distributed into 20 tanks. For data four orthogonal contrasts were applied: control diet versus PSM diets; control diets versus PPCPS diets; control versus other diets; PSM diets versus PPCPS diets. The results indicated that the fish fed PSM diets had lower weight gain when compared to either control diet or PPCPS. The PPCPS do not affect growth and protein efficiency ratio. Lower albumin contents were found for the control diet fish for the contrasts control diet versus PPCPS diet and control diet versus other diets. The hepatic ALAT enzyme activity was higher in the fish fed the control diet (P < 0.05). The hepatic ALP was most active in fish that received the PPCPS diets, when comparing control diet versus PPCPS diets and control diet versus other diets. The hepatosomatic index was higher for fish fed the PPCPS. Our results indicated that PPCPS presents relevant nutritional quality for fish and can replace the fish meal protein up to 50 % without affecting growth, PER and intermediate metabolites in silver catfish.

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Acknowledgments

The authors would like to thank Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—National Counsel of Technological and Scientific Development) for the research yield scholarship (Leila Picolli da Silva), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES—Coordination of Improvement of Higher Education) for the Doctoral degree scholarship (Naglezi de Menezes Lovatto) and Ritter Alimentos company for donating the pumpkin seeds.

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Authors and Affiliations

  1. Departamento de Zootecnia, Universidade Federal de Santa Maria, Rio Grande do Sul. Av. Roraima nº 1000, Cidade Universitária, Bairro Camobi, Santa Maria, RS, 97105-900, Brazil

    Naglezi de Menezes Lovatto, Fernanda Rodrigues Goulart, Silvandro Tonetto de Freitas, Patricia Inês Mombach, Bruno Bianch Loureiro, João Radünz Neto & Leila Picolli da Silva

  2. Departamento de Tecnologia e Ciência dos Alimentos, Universidade Federal de Santa Maria, Rio Grande do Sul. Av. Roraima nº 1000, Cidade Universitária, Bairro Camobi, Santa Maria, RS, 97105-900, Brazil

    Ana Betine Beutinger Bender

  3. Departamento de Farmácia Industrial, Universidade Federal de Santa Maria, Rio Grande do Sul. Av. Roraima nº 1000, Cidade Universitária, Bairro Camobi, Santa Maria, RS, 97105-900, Brazil

    Aline Augusti Boligon

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  1. Naglezi de Menezes Lovatto
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Lovatto, N.M., Goulart, F.R., de Freitas, S.T. et al. Nutritional evaluation of phosphorylated pumpkin seed (Cucurbita moschata) protein concentrate in silver catfish Rhamdia quelen (Quoy and Gaimard, 1824). Fish Physiol Biochem 41, 1557–1567 (2015). https://doi.org/10.1007/s10695-015-0107-0

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